Find and Purchase TOPO TA Cloning Kit with PCR2. 1 TOPO Products at Invitrogen Life Science Technologies. v Kit Contents ShippingStorage The pYES2. 1 TOPO TA Expression Kit is shipped on dry ice. Each kit contains a box with pYES2. 1 TOPO TA Cloning reagents (Box 1) and a box with TOP10F One Shot chemically competent cells (Box 2). Store Box 1 at 20C and Box 2 at 80C. pENTR Directional TOPO Cloning Kits to 2: 1 molar ratio of PCR product: TOPO Technology with Clonase II manual which is available for downloading from www.
invitrogen. com or by contacting Technical Service (see page 35). continued on next page. 2 Overview, continued This PCR Cloning Kit contains an optimized Cloning Mix containing a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background.
Certificate of Analysis, data card or product manual. pCR2. 1TOPO (linearized) Linearized vector for TOPO TA cloning of PCR products. How It Works The plasmid vector (pCRIITOPO or pCR2. 1TOPO) is supplied linearized with: Single 3 thymidine (T) overhangs for TA Cloning Topoisomerase covalently bound to the vector (referred to as" activated" vector) I did cloning using TOPO TA Cloning Kit with PCR2. 1 TOPO, I inoculated 100 ul on Ampicilin plates, but nothing came.
so what could be happened? v Kit Contents and Storage Shipping and Storage The TOPO TA Cloning Kits for Sequencing are shipped on dry ice. Each kit contains a box with TOPO TA Cloning reagents (Box 1) and a box with One Shot Competent E.
coli (Box 2). Store Box 1 at 20C and Box 2 at 80C. TOPO TA Cloning Kit, with pCR2. 1TOPO, One Shot TOP10 Chemically Competent E. coli, and PureLink Quick Plasmid Miniprep Kit Please purchase this product by removing it from your onsite supply center. TOPO TA Cloning Kit. Fiveminute cloning of Taq polymeraseamplified PCR products. Catalog numbers (pCR 2.
1TOPO vector) K, K, K4500J10, K, Lets break down the steps needed for TOPO cloning: 1. Create Your PCR Product: Design standard primers (no need to add unique restriction sites on the ends) and amplify your sequence of interest with Taq polymerase using your favorite PCR protocol.